Fiber enhancement and 3d orientation analysis in label-free two-photon fluorescence microscopy

HIGHLIGHTS

  • who: Michele Sorelli from the DepartmentUniversity of have published the research work: Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy, in the Journal: Scientific Reports Scientific Reports of 21/Nov/2022
  • what: In the present study, the accuracy of the Frangi-based fiber orientation analysis pipeline was evaluated at the micron scale, analyzing image slices randomly sampled from a set of TPFM stacks of grey and white matter, and properly rotated within and out of the image plane of the microscopy setup.
  • how: The authors employed two-photon . . .

     

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