St18 specifies globus pallidus projection neuron identity in mge lineage

HIGHLIGHTS

• who: Luke F. Nunnelly from the Single-cell RNA sequencing sample preparation e, embryos generated from a cross of St18+/- x St18+/- parents. Embryos were collected and MGE was micro-dissected into chilled Hibernate A solution. During the dissection, embryo DNA was obtained using the HOTSHOT protocol, to allow for rapid PCR genotyping. St, null and WT MGE were pooled and dissociated separately. Samples were submitted to the Sulzberger Genome Center Single Cell Core at the Columbia University Medical Center for library construction using X Genomics chemistry. Sequencing is run on a X Genomics platform, and analysis . . .
  

   

  

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