HIGHLIGHTS
SUMMARY
Indeed, in the last century, the extraordinary progress made in the investigations of the structure and functions of the brain were mainly due to the epistemological intermingling of chemical anatomy (i.e., histology, histochemistry, immunocytochemistry), new microscopy techniques (e_g, confocal and two-photon microscopy, electron microscopy, atomic force microscopy) and computer-assisted morphometric methods, allowing detailed quantitative analysis of the images yielded by these new techniques. Although the complex relationship between the glial cells and the neurons have been investigated by severa groups, some microglial aspects are still controversial;cells, for example, question neurons . . .
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