Selective posttranslational inhibition of cavβ1-associated voltage-dependent calcium channels with a functionalized nanobody

HIGHLIGHTS

• who: Travis J. Morgenstern from the , cell culture and transfection Human embryonic kidney (HEK293) cells were a kind gift from the laboratory of DrRobert Kass (Columbia University). Low passage , cells were cultured at , u00b0C in DMEM supplemented with, % fetal bovine serum (FBS) and , mg/mL of penicillin-streptomycin. , cell transfection was accomplished using the calcium phosphate precipitation method. Briefly, plasmid DNA was mixed withu03bcL of , M CaCl, and sterile deionized water (to a final volume of , u03bcL). The mixture was added dropwise, with constant tapping to , u03bcL of, x HEPES buffered saline containing (in mM): HEPES, NaCl . . .
  

   

  

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