HIGHLIGHTS
- who: Daniel C. Volke from the Technical University have published the paper: Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS of 30/Nov/2021
- what: The authors demonstrate multiplex genome editing with a streamlined plasmid assembly protocol that renders the reliable cloning of plasmids with >10 genome targets possible. The authors aimed not only at boosting the performance of the base-editing tool in Pseudomonas, but also at developing a protocol for one-step assembly of pMBEC plasmids equipped with multiple . . .
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