Survey of ticks and tick-borne pathogens in wild chimpanzee habitat in western uganda

HIGHLIGHTS

• who: Camille Lacroux from the To avoid external cuticular microbial contaminants, ticks were processed with commercial bleach diluted at, % for , s and then rinsed for , min in three successive baths of DNA-free water following a published protocol [56]For each tick specimen, total DNA was extracted from whole crushed body using a genomic DNA extraction kit according to the manufacturer`s instructions (DNeasy Blood and Tissue extraction kit, Qiagen, Hilden, Germany). Each individual DNA extract was then tested for the presence of piroplasmid and bacterial pathogens using distinct protocols. To detect tick-borne piroplasmids, each individual . . .
  

   

  

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