HIGHLIGHTS
SUMMARY
Artificial insemination with chilled sperm is a reproductive biotechnology often used in horse breeding (Olaciregui et_al, 2014). Maintaining the acceptable sperm quality after cooling, which affects the frequency of pregnancy, remains an urgent problem at the present time (Baishya et_al, 2015; Pathak et_al, 2020; Ugur et_al, 2019). L-carnitine may exhibit antioxidant, anti-inflammatory and anti-apoptotic effects in various pathophysiological conditions (Chiu et_al, 2004; Surai, 2015). Spermatozoa are the source of NO and the constitutive forms of NOS appear to be involved in sperm motility, condensation and acrosomal response (Herrero and Gagnon, 2001 . . .
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