Activated cd90/thy-1 fibroblasts co-express the δ133p53β isoform and are associated with highly inflamed rheumatoid arthritis

HIGHLIGHTS

• who: Anna K. Wiles from the Total protein lysates from , cells transiently transfected with either empty vector (Vo), or encoding u0394133p53u03b1, u03b2 or u03b3, as previously described [26] were separated on Bolt, % Bis-Tris Plus Gels (Invitrogen) and transferred onto nitrocellulose membranes, blocked with Odyssey Blocking Buffer, incubated overnight in primary antibody (1:1000) and detected using the secondary antibody IRDye CW goat anti-rabbit IgG (LICOR Biosciences, USA)The membranes were analysed using the Image Studio software (LI-COR Biosciences, USA). The specificity of rabbit p53u03b2 (79.3) and u0394133p, (KJCA133u03b1u03b2u03b3) antibodies (courtesy of Bourdon Laboratory . . .
  

   

  

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