HIGHLIGHTS
- who: Dae D. Chung from the lysis was used on fetal tissue samples to obtain genomic DNA, followed by a rapid qPCR protocol with primers to detect repetitive sequences on X and Y chromosomes to determine the fetal sex [51, ]Male and female sexspecific cultures were generated by pooling dorsal telencephalic cortical neuroepithelial tissues from a single pregnancy by sex. Three separate biological replicates for each fetal sex were generated by collecting and dissociating sex-specified neuroepithelia from three separate pregnancies into single cell suspensions, and maintaining these as non-adherent neurospheres in serum-free mitogenic media . . .
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