HIGHLIGHTS
SUMMARY
Although wastewater-based monitoring of SARS-CoV-2 is already being implemented in practice, there is no unified and standardized method for detecting the virus RNA concentration. Dimitrakopoulos et_al reported high reproducibility and 38-39% viral RNA recovery efficiency for different concentrations and subsequent extraction methods using the Manual Enviro Wastewater TNA kit (Promega, United_States). The aim of this research was to investigate the efficiency of viral RNA recovery, using four of the most common methods for concentrating viral particles from wastewater. The supernatant was used for the subsequent nucleic_acid concentration and extraction steps . . .
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