A novel peptide reagent for investigating disulfide-coupled folding intermediates of mid-size proteins

HIGHLIGHTS

  • who: Nana Sakata and colleagues from the Faculty of Science and Engineering, Kindai University, Kowakae, Higashi-Osaka, Japan have published the Article: A Novel Peptide Reagent for Investigating Disulfide-Coupled Folding Intermediates of Mid-Size Proteins, in the Journal: Molecules 2023, 28, 3494. of /2023/
  • how: The results indicated that the allowed the intermediates to be captured separated on SDS-PAGE and analyzed by RP-HPLC without the occurrence of undesirable disulfide-exchange reactions during the labeling reactions. In this study a maleimide group was employed since the maleimide group reacts with a thiol group . . .

     

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