Examination of gametocyte protein 22 localization and oocyst wall formation in eimeria necatrix using laser confocal microscopy and scanning electron microscopy

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• who: Lele Wang from the The Yangzhou strain of Enecatrix used in this study was originally isolated from a chicken that died from E. necatrix infection in , in Yangzhou, China, as confirmed by microscopic examination and sequence analysis of the internal transcribed spacer region of genomic DNA [26]. This strain has been maintained in our laboratory. The oocysts were periodically propagated in, weekold chickens. Oocysts were isolated and harvested from the feces by salt flotation and centrifugation, sporulated in vitro at , u00b0C, and stored in, .5% potassium dichromate solution at , u00b0C [26]. One-day-old chickens (purchased . . .
  

   

  

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