Programming bacteria for multiplexed dna detection

HIGHLIGHTS

  • who: Yu-Yu Cheng from the Direct detection of target species using cell-based sensors The target strains Ecoli, S. typhimurium, and S. aureus and DNA sensor strains were inoculated from the , u00b0C glycerol stock into LB medium and incubated at , u00b0C with shaking (, rpm) for , h. C. difficile was inoculated in YBHI medium and incubated in an anaerobic chamber (Coy Laboratory). On the next day, cell cultures of sensor and target strain were diluted (, ., each) into a single culture containing , mL LB supplemented with , mM xylose with or without , u00b5g/mL spectinomycin in , mL Falcon tubes . . .

     

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