The igg4 hinge with cd28 transmembrane domain improves vhh-based car t cells targeting a membrane-distal epitope of gpc1 in pancreatic cancer

HIGHLIGHTS

• who: Nan Li from the Cell culture The , (originally from ATCC #CRL-1555) and HEK- T (originally from ATCC #CRL-3216) cell lines were obtained from DrIra Pastan at the NCI., a transfected , cell line stably expressing human GPC1, was made in our lab. , SuperTopflash stable cell line was obtained from Dr. Jeremy Nathans at the Johns Hopkins University. The aforementioned cell lines were cultured in DMEM supplemented with, % FBS, %, glutamine, and, % penicillin-streptomycin at , u00b0C in a humidified atmosphere with, % CO2. Pancreatic cancer cell lines including T, Aspc, (originally from ATCC #CRL-1682), Bxpc, (originally from . . .
  

   

  

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