HIGHLIGHTS
SUMMARY
While these promoters tend to be leaky, they can minimize unwanted vector expression in off-targets and thereby increase in_vivo vector safety, especially when used in combination with capsids with a broad cell specificity. Pioneering work by Girod et_al identified these insertion sites by aligning the AAV2 capsid amino_acid sequence to the ones from canine parvovirus, for which the X-ray_crystal_structure was already available. A standard directed evolution approach for AAV capsid diversification is the insertion of random peptides into the previously identified capsid positions, followed by the use of in_vitro or in_vivo selection . . .
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