Transcription factor binding site orientation and order are major drivers of gene regulatory activity

HIGHLIGHTS

  • who: Ilias Georgakopoulos-Soares from the (UNIVERSITY) have published the Article: Transcription factor binding site orientation and order are major drivers of gene regulatory activity, in the Journal: (JOURNAL)
  • what: Using a massively parallel reporter assay (MPRA) of 209,440 sequences, the authors examine all possible pair and triplet combinations, permutations and orientations of eighteen liver-associated transcription factor binding_sites (TFBS). This study provides several important observations on regulatory grammar.
  • how: In previous work the authors identified widespread transcriptional strand asymmetries in the orientation of TFBSs across human transcribed regions10 a research area . . .

     

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