P53 promotes peroxisomal fatty acid β-oxidation to repress purine biosynthesis and mediate tumor suppression

HIGHLIGHTS

• who: Jianhong Zhao from the Plasmids and constructs The open reading frames of human TP53, VDR, ACOX1, ATIC, and KAT B or mouse Atic were amplified and cloned into pHAGE-CMV-Flag, pCMV-HA, pLKO-GFP or pAAV-EF1a-GFPKATs were kindly provided by Prof. HongBing Shu (Wuhan University, China) [53]. Mutations in the ATIC cDNA sequences were generated by overlap extension PCR. Human TP53, VDR, ACOX1, ATIC, and KAT B or mouse Atic shRNAs were synthesized by from GENECREATE (Wuhan, China), subsequently annealed, and inserted into the pLKO.1-puro vector. Human VDR sgRNAs were ordered . . .
  

   

  

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