Segregated cation flux by tpc2 biases ca2+ signaling through lysosomes

HIGHLIGHTS

• who: Yu Yuan from the (all from Invitrogen) at , u00b0C in a humidified atmosphere with, % CO These lines are not commonly misidentified. Cells were passaged with trypsin. Cells were plated onto coverslips coated with poly-L-lysine (20, u03bcg/mL, Sigma) for epifluorescence imaging and electrophysiology or with poly-D-lysine (, u03bcg/mL, Sigma) for TIRF imaging. For vacuolar patch clamp measurements, cells were treated with apilimod (, u00b5M) for , h to , h to enlarge endo-lysosomal organelles. For plate reading, cells were plated onto opaque-walled , well microplates (Corning). Pancreatic acinar cells were obtained from male, weeks . . .
  

   

  

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