Flip-based autophagy-detecting technique reveals closed autophagic compartments

HIGHLIGHTS

• who: Hajime Tajima Sakurai from the DepartmentDental University have published the paper: FLIP-based autophagy-detecting technique reveals closed autophagic compartments, in the Journal: Scientific Reports Scientific Reports of 13/09/2022

SUMMARY

If there are closed autophagic compartments (autophagosomes and autolysosomes) within the non-bleached area, the fluorescent proteins incorporated into these structures are evaded from streaming, so that they are detectable as fluorescent puncta (Fig 1A). Upon starvation, F ­ LAD+ puncta were observed and increased time-dependently, and many of them colocalized with GFP-LC3+ puncta (Fig 2A,B, and Suppl . . .

 



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