HIGHLIGHTS
- who: Jin Zhang from the Trp73+/- mice were generated as described previously [19]E13+/- mice were generated by the Mouse Biology Program at University of California at Davis. All animals and use protocols were approved by the University of California at Davis Institutional Animal Care and Use Committee. To generate WT and, /- MEFs, +/-mice were bred and MEFs were isolated from, ., to, ., postcoitum (p.c.) mouse embryos as described previously [20]. MEFs were cultured in DMEM supplemented with, % FBS (HyClone), u03bcM u03b2-mercaptoethanol, and, u00d7 non-essential amino acids (NEAA) solution (Cellgro). The genotyping primers for the . . .
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