HIGHLIGHTS
SUMMARY
The authors also demonstrated that a kissing-loop interaction between trans-molecules produced a unique G:U wobble base_pair in an appropriate position, corresponding to the acceptor stem of tRNA, which can be a critical recognition site for alanyl-tRNA synthetase (AlaRS). Non-fluorescence-labeled RNAs were prepared by transcription using T7 RNA_polymerase. RNA transcription was performed at 37 ◦ C for 16 h in a reaction mixture containing 40 mM Tris-HCl (pH 8.0), 10 mM dithiothreitol, 2 mM spermidine, 8 mM MgCl2, and 2.5 mM each of NTP, template DNA (~0 . . .
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