Regulatory mechanisms of lipopolysaccharide synthesis in escherichia coli

HIGHLIGHTS

• who: Sheng Shu from the KdtA (residues, ) were fused to the cytoplasmic region of LapB (aa, ) by overlap PCRSequences of primers are listed in Supplementary Table, . The gene or gene truncations were then cloned to linearized vector using Gibson Assembly kit (New England BioLabs). All the constructs were confirmed by DNA sequencing from the Keck DNA sequencing facility at Yale University. Overexpression and purification of FtsH:, terminally Histagged FtsH in pETduet vector was transformed into E. coli strain , StarTM (DE3) pLysS. Cells were grown at , u00b0C in Terrific broth (TB) medium with , u03bcg/ml ampicillin and, ., u03bcg . . .
  

   

  

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