The e3 ligase rnf5 restricts sars-cov-2 replication by targeting its envelope protein for degradation

HIGHLIGHTS

• who: Zhaolong Li from the were synthesized and inserted into SalI/BamHI of , by the Comate Bioscience company (Changchun, CHN)Mutants and truncations of, HA were constructed by PCR with primers listed in Supplementary Table, ., Flag (codon optimized in accordance with the human genome) carrying an Flag tag was inserted into pCDNA B and, GFP (N protein was inserted into pEGFP-C1) (BD Biosciences Clontech, catalog no., ) of SARS-Co, were gifts of Professor Wang Peihui (Shandong University)., Human , fragments carrying a Flag tag at the N terminal end were amplified from cDNAs of Caco, cells, and . . .
  

   

  

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