HIGHLIGHTS
SUMMARY
Molecular markers help deepen the understanding of GC subtypes (Bijlsma et_al, 2017; Zhao et_al, 2019), and with the technological advances in highthroughput sequencing, the focus on tumor heterogeneity is driving progress in precision medicine (Zeng and Jin, 2021) simultaneously. Single-cell RNA sequencing (scRNA-seq) is a potent tool to obtain complete RNA transcripts at the level of single cells by RNA extraction, reverse transcription, amplification, and sequencing (Tang et_al, 2009; Ramskold et_al, 2012; Picelli et_al, 2014). It shows clear superiority in studying the diversity of tumor cell lineages and predicting interactions between cancer . . .
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