Patients with isolated rem-sleep behavior disorder have elevated levels of alpha-synuclein aggregates in stool

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• who: Anja Schaffrath from the groupFor synthesis of protein-conjugated SiNaPs, % of the possible binding sites were functionalized by adding protein to dispersed SiNaPs. Protein-coupled SiNaPs were subjected to ultrasonication for , min prior to each use. The same batches of u03b1synuclein or Au03b2 SiNaPs were used for all experiments. To serve as a calibration standard, the stock solution of u03b1-synuclein SiNaPs was diluted in extraction buffer (EliA Calprotectin , Extraction Buffer (Phadia, Uppsala, Sweden) with, u00d7 protease inhibitor cocktail (cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, Basel, Switzerland)) in a half-logarithmic dilution series from , to . . .
  

   

  

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