Disease-associated mutations within the yeast dnajb6 homolog sis1 slow conformer-specific substrate processing and can be corrected by the modulation of nucleotide exchange factors

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• who: Ankan K. Bhadra from the The yeast strains used in this study are derived from Saccharomyces cerevisiae, (ade1, his3-u0394, leu2-3, trp1, ura, )Yeast cells were grown and manipulated using standard techniques68. As indicated, cells were grown in rich media YPD (1% yeast extract, % peptone, % dextrose) or in synthetic defined (SD) media (0.67% yeast nitrogen base without amino acids, % dextrose) lacking specific nutrients to select for appropriate plasmids. Wild-type (WT) yeast harboring the s. d. medium [RNQ+] variant and the [rnq-] control strain were kindly provided by Dr. Susan Liebman (University of Nevada . . .
  

   

  

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