Motif-targeting phosphoproteome analysis of cancer cells for profiling kinase inhibitors

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  • who: Kosuke Ogata et al. from the Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan have published the Article: Motif-Targeting Phosphoproteome Analysis of Cancer Cells for Profiling Kinase Inhibitors, in the Journal: Cancers 2023, 15, 78. of /2023/
  • what: The authors propose a phosphoproteomic subset approach in which phosphoproteome samples after phosphopeptide enrichment are completely dephosphorylated and then selectively phosphorylated with specific kinases. In the present study, the tyrosine kinase-based motif-targeting approach identified over three times more tyrosinecontaining phosphopeptides than the conventional global phosphoproteomic approach.
  • how: The authors hypothesized . . .

     

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