Tak-715 alleviated il-1β-induced apoptosis and ecm degradation in nucleus pulposus cells and attenuated intervertebral disc degeneration ex vivo and in vivo

HIGHLIGHTS

• who: Kun Wang from the Rat NPCs were provided as a gift by DrChen Di of Rush University Medical Center (Chicago, IL, USA) [13]. After SD rats (male, weight, g) were euthanized, NP tissues were harvested from caudal intervertebral discs, and then digested in, .2% pronase for , h at , u00b0C, followed by, .5% collagenase II digestion for , min at , u00b0C. Then, the digested tissues were washed and cultured in Dulbecco`s modified Eagle`s medium (DMEM) containing, % fetal bovine serum (FBS) and, % penicillin-streptomycin at , u00b0C. The NP cells were cultured in DMEM with, % C u00ad , at . . .
  

   

  

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