“proteotranscriptomic analysis of advanced colorectal cancer patient derived organoids for drug sensitivity prediction”

HIGHLIGHTS

• who: Federica Papaccio from the PDOs pellets were lysed in UTC buffer (, M urea, M thiourea and, % CHAPS) and the protein concentration was measured with the RC DC Protein Assay (BioRad)A pooled sample containing , u00b5g of protein from each sample was used to prepare the peptide library. For the SWATH analysis, u00b5g of protein from each sample were used. Samples were denatured at , u00b0C for , min in sample buffer and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Pooled samples were resolved in gel and lanes were cut into five pieces. Gels containing individual . . .
  

   

  

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