Atf6 prevents dna damage and cell death in colon cancer cells undergoing er stress

HIGHLIGHTS

• who: Rossella Benedetti from the , O) were a kind gift from BVogelstein (Johns Hopkins University, Baltimore, MD). Cells were maintained in DMEM (PAN-Biotech, Aidenbach, Germania) supplemented with, % Fetal Bovine Serum (FBS) (Corning, Corning, NY, USA), glutamine (, u03bcg/ml) (Aurogene, Rome, Italy), streptomycin and penicillin (, U/ml) (Aurogene) at , u00b0C in a, % , incubator. Cells were always detached using Trypsin-EDTA solution (Aurogene). Cells were plated in, well plates at a density of , cells/well in , ml and treated the following day. The sensitivity of the RKO and , cells to DPE was previously evaluated [12], while to analyze . . .
  

   

  

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