Anti-biofilm activity of pseudomonas fluorescens culture supernatants on biofilm formation of staphylococcus epidermidis

HIGHLIGHTS

• who: Euna Choi from the Sepidermidis, kindly donated by Dr. Paul Fey (University of Nebraska Medical Center), was cultured in tryptic soy broth (TSB) at , u00b0C. P. fluorescens Pf, strain was a gift from Dr. George O`Toole (Dartmouth University) and was maintained in Luria-Bertani broth (LB) at , u00b0C. To prepare the CS, an overnight P. fluorescens culture was used to inoculate , ml LB (starting u00adOD660=0.05). The culture was grown at , u00b0C with agitation to the early-stationary phase u00ad(OD660=0.8), centrifuged at , u00b0C, , g for , min, passed through au00b5m filter system . . .
  

   

  

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