Lncrna xist regulates breast cancer stem cells by activating proinflammatory il-6/stat3 signaling

HIGHLIGHTS

• who: Yuxi Ma from the ALDEFLUOR assay, cell labeling and flow cytometry The ALDEFLUORu2122 (STEMCELL Technologies Inc, Vancouver, Canada) kit was used to detect aldehyde dehydrogenase (ALDH) enzymatic activity following manufacturer`s instructionsBriefly, single cells suspended at , u00d7 , cells/mL in Aldefluor buffer are incubated with, uL of BODIPY-aminoacetaldehyde (BAAA) for , min at , u00b0C, and , uL of diethylaminobenzaldehyde (DEAB) was added along with BAAA as negative control. Aldefluor-labeled cells were resuspended in Aldefluor buffer containing , u00b5g/mL of, u2032, diamidino-2phenylindole (DAPI, Sigma) to discriminate live from dead cells. To detect MCSC-like cells in . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.