Pathogens, reservoirs, and vectors involved in the transmission of vector-borne and zoonotic diseases in a colombian region

HIGHLIGHTS

• who: Maru00eda Cristina Carrasquilla from the Determination of bloodu2011meal sources in vectors Blood-meal analysis was performed to find out if potentially infected vectors fed on humans or other animalsInsect DNA templates were tested with primers for mammalian cytochrome B [29] and for human beta-globin gene [30]. For mammalian primer PCR, u03bcl total DNA (, to , ng/u00b5l) were mixed with the following reagents:, ., u03bcl of , u00d7 GoTaq Green Master Mix (Promega, ), u03bcM of forward and reverse primers to a final reaction volume of , u03bcl. The thermo cycling conditions consisted of one cycle of , min at , u00b0C . . .
  

   

  

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