HIGHLIGHTS
SUMMARY
Currently, there are limited tools to study SUN oligomerization and SUN-KASH protein interaction in_vivo (Hennen et al, 2018). Conversely, an experimentally validated intermolecular disulfide_bridge between cysteine residues in SUN and KASH domains has been described that is dispensable for KASH binding but required for maximal force transmission (Jahed et al, 2015; Cain et al, 2018). Upon co-staining with both SUN2 antibodies, the authors found that Sun2C failed to recognize SUN2 in the presence of DN-KASH compared with mCherry alone expressing cells (Fig 1D) with significantly reduced Sun2C intensity (Fig 1F, see . . .
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