Selection and validation of reference gene for rt-qpcr studies in co-culture system of mouse cementoblasts and periodontal ligament cells

HIGHLIGHTS

  • who: Jiawen Yong from the (UNIVERSITY) have published the research: Selection and validation of reference gene for RT-qPCR studies in co-culture system of mouse cementoblasts and periodontal ligament cells, in the Journal: (JOURNAL)
  • what: The authors aimed to identify a set of reli‑ able reference genes suitable for RT-qPCR studies for prospective co-culture systems of OCCM-30 and SV-PDL cells.
  • how: For every RT-qPCR 20 μL volume reaction the authors used 8 μL DNase-free water (Sigma-Aldrich) 10 μL SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad . . .

     

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