Selective isotope labeling for nmr structure determination of proteins in complex with unlabeled ligands

HIGHLIGHTS

  • who: Konstantinos Tripsianes from the CEITEC-Central European Institute of Technology, Masaryk University, Kamenice, Brno, Czech Republic have published the article: Selective isotope labeling for NMR structure determination of proteins in complex with unlabeled ligands, in the Journal: (JOURNAL)
  • what: The authors report an optimized protocol for NMR structure calculation of proteins, where ligands or covalently attached modifications cannot be readily obtained in isotope-labeled form.
  • future: The basic principles should enable to assess the complexity of the system adapt the individual steps accordingly and supplement the workflow with additional experiments if required . . .

     

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