Cooperation of pd-1 and lag-3 in the exhaustion of cd4+ and cd8+ t cells during bovine leukemia virus infection

HIGHLIGHTS

• who: Tomohiro Okagawa from the infection was diagnosed at Graduate School of Veterinary Medicine, Hokkaido UniversityBriefly, genomic DNA was extracted from whole blood using the Wizard Genomic DNA Purification kit (Promega, Madison, WI, USA). BLV was detected by nested PCR targeting the BLV long terminal repeat region (LTR) using the following primers: BLV-, ′-TGT ATG AAA GAT CAT GCC GAC-3′ and BLV-, ′-AAT TGT TTG CCG GTC TCT-3′ for the initial PCR, and BLV-, ′-GAG CTC TCT TGC TCC CGA GAC-3′ and , ′-GAA ACA AAC GCG GGT GCA AGC CAG3′ for the second PCR . . .
  

   

  

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