Overlapping and opposing functions of g protein-coupled receptor kinase 2 (grk2) and grk5 during heart development*

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  • who: Melanie Philipp from the EXPERIMENTAL PROCEDURES Zebrafish Strains and Husbandry-Zebrafish were kept under standard conditions in a, h light and, h dark cycleWild-type fish of EK and AB strains were used. All procedures and experiments were carried out according to animal protocols approved by local authorities at Duke University and Ulm University. Zebrafish Microinjection-Fertilized zebrafish eggs were injected at the, cell stage using pulled glass capillaries (World Precision Instruments, Sarasota, FL) and an Eppendorf Femtojet Microinjector (Eppendorf, Hamburg, Germany). Embryos were then allowed to develop to the indicated stages while being incubated at . . .

     

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