Pore helices play a dynamic role as integrators of domain motion during kv11.1 channel inactivation gating*

HIGHLIGHTS

• who: Matthew D. Perry from the EXPERIMENTAL PROCEDURES Molecular Biology-Kv, cDNA (a gift from G. Robertson, University of Wisconsin, Madison, WI) was subcloned into a pBluescript vector containing the, ⬘-untranslated region (UTR) and, ⬘-UTR of the Xenopus laevis ␤-globin gene (a gift from R. Vandenberg, University of Sydney). Mutagenesis of Kv11., cDNA was performed using the QuikChange method (Agilent Technologies, Santa Clara, CA) and confirmed by DNA sequencing. Linearization of the plasmid was performed using BamHI-HF (New England Biolabs, Ipswich, MA), and cRNA was in vitro transcribed using the mMESSAGE mMACHINE kit (Ambion). Electrophysiology-Female . . .
  

   

  

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