Lipolysis response to endoplasmic reticulum stress in adipose cells*

HIGHLIGHTS

• who: Jingna Deng from the tion at , ⫻ g for , min and then plated and differentiated into adipocytes for , days in serum-free DMEM/, (1:1) supplemented with , ␮g/ml insulin, ␮M biotin, and , pM triiodothyronine, as we described previously (15, )At day, the differentiating adipocytes were transferred to phenol red-free and serum-free DMEM and incubated for , h before experiments. Evaluation of Lipolysis by Glycerol Assay-Full hydrolysis of one triglyceride in adipocytes produces three fatty acids and one glycerol molecule, which are then released into the medium. Thus, we measured glycerol content in the medium . . .
  

   

  

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