The proline⠍arginine repeat protein linked to c9-als/ftd causes neuronal toxicity by inhibiting the dead-box rna helicase-mediated ribosome biogenesis

HIGHLIGHTS

• who: Hiroaki Suzuki from the , motor neuron cell, a hybrid cell line established from a mouse neuroblastoma cell line and mouse embryo spinal cord cells, was a kind gift from DrNeil Cashman (University of Toronto). , cells were derived from our laboratory depository. The absence of mycoplasma contamination of these cell lines was validated using MycoAlert™ Mycoplasma Detection Kit (Takara). , cells and , cells were grown in Dulbecco's modified Eagle's medium (DMEM) (Wako), supplemented with, % of fetal bovine serum (FBS) (GE Healthcare UK Ltd, Buckinghamshire, England) and antibiotics (Thermo Fisher Scientific). Transfection was performed using Lipofectamine (Thermo . . .
  

   

  

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