Adjacent sequence controls the response polarity of nitric oxide-sensitive sp factor binding sites*

HIGHLIGHTS

• who: Jianhua Zhang from the Reagents-Phorbol, myristate, acetate (PMA), nitroso-Nacetylpenicillamine (SNAP), Bt2cAMP, and , were purchased from Calbiochem (San Diego, CA)N-Acetylpenicillamine (NAP) was obtained from Sigma. The enhanced chemiluminescence immunoblot detection system was purchased from Amersham Biosciences. Polyvinylidene difluoride membranes, , % Tris glycine gels, and, % DNA retardation gels were purchased from Novex (San Diego, CA). Polyclonal anti-Sp1, anti-Sp3, anti-c-Jun/AP1, and anti-, antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA) or Upstate Biotechnology Inc. (Lake Placid, NY). Monoclonal anti-eNOS and polyclonal anti-mouse IgG conjugated to horseradish peroxidase . . .
  

   

  

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