Translocation of full-length pseudomonas exotoxin from endosomes is driven by atp hydrolysis but requires prior exposure to acidic ph*

HIGHLIGHTS

• who: Marie-Pierre Taupiac from the Cells and Reagents-Mouse T lymphocytes (BW, ) and , fibroblasts were grown as described (19, )Purified PE was purchased from the Swiss Serum and Vaccine Institute (Bern, Switzerland). Molecular biology enzymes were from Promega or Life Technologies, Inc. DNA sequencing was performed using a silver sequence DNA-sequencing system (Promega). Proteins were radiolabeled with I (Amersham) using the iodogen method (21). Upon I labeling of transferrin conjugates, transferrin incorporated have published the research: Translocation of Full-length Pseudomonas Exotoxin from Endosomes Is Driven by ATP Hydrolysis but Requires Prior Exposure to Acidic . . .
  

   

  

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