HIGHLIGHTS
- who: Kazuko Fujiwara from the analysis indicated the presence of a single transcript of , kilobases. (v) A specific mRNA for LipTI could not be detected by RT-PCR analysis. We cloned and expressed a cDNA encoding mammalian lipoyltransferase for the first time. Purification of lipoyltransferase from animal liver is laborious because of the low content of the enzyme. Here we present a simple purification method to obtain milligram quantities of recombinant bovine lipoyltransferase II. The recombinant enzyme showed the same properties as the native enzyme. The availability of cDNA clone coding for lipoyltransferase should facilitate studies of . . .
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