Adipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles

HIGHLIGHTS

  • who: Alice S. Ferng from the ASCs were isolated from freshly excised human subcutaneous adipose tissue following methods adapted from Estes et_al (2010)Donors (n=12) were both male and female, between , and , years of age. All samples were obtained as surgical discards with approval from the University of Arizona Internal Review Board (IRB approval numbers:, and , ) from the adipose tissue surrounding the following regions: abdominal (subcutaneous), knee (peri-patellar), shoulder, pericardium, and thymus. In brief, adipose tissue was minced and washed extensively with X PBS +, % antibiotic-antimycotic (Thermo Scientific, Waltham, MA), and then incubated at , °C . . .

     

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