Limitation in use of heterologous reporter genes for gene promoter analysis

HIGHLIGHTS

• who: Kezhong Zhang from the Materials-Restriction enzymes and DNA modification enzymes were purchased from Invitrogen and New England BiolabsRadioactive nucleotides, [␣- P]dCTP, were obtained from Amersham Biosciences. Mouse anti-hPC monoclonal antibody and rabbit polyclonal anti-hPC antibodies were purchased from Celsus Laboratory. Horseradish peroxidase-linked goat anti-rabbit IgG was purchased from Invitrogen. Anti-hFIX polyclonal and monoclonal antibodies for enzyme-linked immunosorbent assay (ELISA) were obtained from Hematologic Technologies Inc and Enzyme Research Laboratory, respectively, as previously described (12). Media, fetal calf serum, penicillin, and streptomycin for mammalian cell cultures were obtained from Invitrogen . . .
  

   

  

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