Tumour-derived sparc drives vascular permeability and extravasation through endothelial vcam1 signalling to promote metastasis

HIGHLIGHTS

• who: Mé and lanie Tichet from the )Melanoma cells were pulsed with , mM CellTracker Green CMFDA for , min and collected by HyQTase treatment. Fluorescent dye-labelled cells were then allowed to adhere onto the endothelial cell monolayer for , h. Non-adherent cells were removed by washing with PBS supplemented with , mM CaCl, and , mM MgCl, and fluorescence was measured. Data are expressed as the percentage of adherent cells per total cells. Detection of ROS by flow cytometry. ROS levels were measured using the redox-sensitive dye CM-H DCFDA (Molecular Probes, Life Technologies). The green fluorescence . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.