HIGHLIGHTS
- who: Anton A. Komar from the dium containing, % Difco yeast nitrogen base, % ammonium sulfate, % glucose (or galactose) and supplemented with the appropriate amino acids or complete (YEPD) medium (17). Transformation was performed using the lithium acetate method (18). For the polysome expression analysis of HA-tagged eIF A, yeast were grown in complete medium with, % galactose. Plasmids-The pTB328_y_ A shuttle vector bearing yeast eIF A (fused to an HA epitope) under control of the GAL promoter has been previously described (14). The YCplac, (CEN, LEU2) vector bearing yeast eIF A under its own promoter was produced . . .
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