The lactose operon from lactobacillus casei is involved in the transport and metabolism of the human milk oligosaccharide core-2 n-acetyllactosamine

HIGHLIGHTS

• who: Gonzalo N. Bidart from the DNA was isolated from Ecoli by using the kit illustra plasmidPrep Mini Spin (GE Healthcare, UK). Standard methods were used for recombinant DNA techniques, and PCR reactions were carried out with the Expand High Fidelity PCR System (Roche). The DNA sequencing reactions were performed by the Central Service of Research Support of the University of Valencia (Spain). Specific primers hybridizing within the proper DNA fragments and universal primers were used for sequencing. The analysis of DNA sequences was performed with the aid of the DNAMANsoftware package (Lynnon BioSoft) and sequence similarities . . .
  

   

  

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