The effects of zinc amino acid complex supplementation on the porcine host response to lawsonia intracellularis infection

HIGHLIGHTS

• who: Fernando L. Leite from the To investigate the antibody response of the pigs to Lintracellularis, serum samples were collected by venipuncture of the cranial vena cava at, and , dpi. Serum samples were serially diluted from, :, to, :, in phosphate-buffered saline containing, .5% fetal bovine serum, .0% rabbit serum and, .08% tween , and screened for antibodies against L. intracellularis using the immunoperoxidase monolayer assay (IPMA) [18]. To measure shedding of L. intracellularis, fecal samples were collected at the same time points and DNA was extracted. Real time PCR was then used to estimate the quantity of L . . .
  

   

  

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